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 acc0
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 Detaches
Adherent Cells in Minutes
Dissociates
Tissues for Primary Cell Culture
Gentle
Cell Detachment for Maximum Cell
Viability
Highest
Plating Efficiency
Cost
Effective and Ready to Use |
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CELL
DETACHMENT
Human MG63 fibrosarcoma cells
cultured on tissue culture treated
dishes in DMEM + 10% FBS were
treated with ACCUTASE. Treatment
results in
Rapid
cell detachment,
A
single cell suspension,
High
viability.
ACCUTASE is gentle on cells; viability
was 97 ± 3% even after 45 minutes
in ACCUTASE.
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Accutase
has been developed to meet the
most demanding needs for gentle
and effective detachment of adherent
cells.
Accutase
has been proven effective in detaching
primary fibroblasts, endothelial
cells, neurons, tumor cell lines,
and insect cells.
Accutase
is recommended for use with SmartPlastic*
adhesion cell culture plasticware.
Accutase
gently and rapidly dissociates
tissues for cell isolation and
propagation.
Accutase
combines protease and collagenolytic
activities which maximizes its
versatility for cell detachment
and tissue dissociation.
Accutase
does not contain mammalian or
bacterial-derived products.
Accutase
is available in a ready-to-use
formulation, quality controlled
for in vitro cell culture applications.
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The Accutase Advantage
Compare Accutase with your current
method of cell detachment:
|
Accutase
|
Others |
|
| yes |
no |
Easy to use, cost effective |
| yes |
no |
Effective for most cell
culture applications |
| yes |
no |
Gentle to cells |
| yes |
no |
Tissue dissociation for
primary cell culture |
| yes |
no |
Non-prokaryote |
| yes |
no |
Non-mammalian |
| yes |
no |
No neutralization required |
| yes |
no |
Effective at removing
cells from SmartPlastic |
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Testing: Accutase
has been extensively tested in
research and biopharmaceutical
laboratories.
Cell Lines: Accutase
has been shown effective on: fibroblasts,
keratinocytes, vascular endothelial
cells, hepatocytes, vascular smooth
muscle cells, hepatocyte progenitors,
primary chick embryo neuronal
cells, bone marrow stem cells,
adherent CHO and BHK cells, macrophages,
293 cells, L929 cells, immortalized
mouse testicular germ cells, 3T3,
Vero, COS, HeLa, NT2, MG63, M24
and A375 metastatic melanoma,
gliomas U251 and D54, HT1080 fibrosarcoma
cells, and Sf9 insect cells.
Application: Accutase
performs exceptionally well in
detaching cells for:
analysis of cell surface markers,
virus growth assay,
quiescence assays by serum starvation,
transformation assays by oncogene
transfection,
neural crest cell migration assays,
cell proliferation,
cell haptotaxsis,
tumor cell migration assays,
routine cell passage,
production scale-up (bioreactor),
flow cytometry.
Formulation:
Accutase has both protease and
collagenolytic activity. Each
lot of Accutase is individually
formulated to meet quality control
standards for effective cell detachment.
Accutase is supplied in Dulbecco's
PBS containing O.5mM EDTA·4Na
and phenol red.
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 acc02
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Dissociates
Clumped Cells in Minutes
Results
in Single Cell Suspension
Gentle
Cell Disaggregation for Maximum
Cell Viability
Yields
Accurate, Reproducible Cell Counts
Cost
Effective
Available
Ready to Use |
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COUNT
Various constructs of genetically
engineered
CHO
cells
BHK
cells
a
hybridoma
were grown in suspension in serum-free
or protein-free medium.
Representative cell aliquots were
treated with an equal volume of
PBS or ACCUMAX and incubated for
5 minutes at 37°C.
Cell number was then determined
with a Coulter Counter.
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Accumax
has been developed to meet the
most demanding needs for an effective
cell aggregate dissociation solution.
Accumax has been proven effective
in dissociating clumped cells
in suspension cultures of hybridomas,
CHO, BHK, 293, and others.
Accumax
gently and rapidly dissociates
cell clumps to yield single cell
suspensions for accurate and reproducible
cell counts.
Accumax
combines protease, collagenolytic,
and DNase activities which maximizes
its versatility for cell aggregate
dissociation.
Accumax does not contain mammalian
or bacterial-derived products.
Accumax is available as a ready-to-use
liquid, quality controlled for
in vitro cell culture applications.
|
The Accumax Advantage
Compare Accumax with your current
method of disaggregation:
|
Accutase
|
Others |
|
| yes |
no |
Cell aggregate dissociation
for accurate cell counts |
| yes |
no |
Easy to use, cost effective |
| yes |
no |
Effective for most suspension
cell culture applications |
| yes |
no |
Gentle to cells |
| yes |
no |
Non-prokaryote |
| yes |
no |
Non-mammalian |
| yes |
no |
Results you can count
on |
|
Testing: Accumax
has been extensively tested in
research and biopharmaceutical
laboratories.
Cell Lines: Accumax
has been shown effective in dissociating
cell clumps in a variety of cell
lines including hybridomas, CHO,
BHK, 293, COS and Sf9 cells.
Application: Accumax
performs exceptionally well in
dissociating cell clumps for
cell counting,
viral transfection assays,
cell sorting,
flow cytometry
bioreactor scale-up.
Formulation: Accumax
has protease, collagenolytic and
DNase activity. Each lot of Accumax
is individually formulated to
meet quality control |
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